Or maybe the person in question decides they would rather stay this way, in which case see the Second Law of Gender-Bending. Or maybe the phlebotinum works by copying the X chromosome and deleting the Y, and now there's no way to recreate it. Perhaps our protagonist now has gender-based superpowers and needs them to save the day. Maybe the phlebotinum is reversible but a Phlebotinum Breakdown crops up at just the wrong moment. This can be justified by the plot in any number of ways. One might think that the same Applied Phlebotinum that can change a male into a female should just as easily be able to do the opposite, but that's rarely the case in fiction. Not only are male-to-female Gender Benders a lot more common than their female-to-male counterparts, they are also more likely to stay transformed. "Once someone has been made female, circumstances will conspire to keep them that way."
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